PrimeRoot (2023)
Nature Biotechnology reported PrimeRoot, which coupled enhanced prime editors with Cre recombinase to insert up to 11.1 kb into rice. Its best reported efficiency was 6.3% for a blast-resistance cassette conferring PigmR.
Established that double-strand-break-free large insertions were possible in plants, but at low efficiency.
Became the benchmark PrimeStack had to beat.
PrimeStack is PrimeRoot's direct successor, swapping Cre for the unidirectional Bxb1 integrase and roughly septupling efficiency.
